Optimization of lung tissue pre-treatment by bead homogenization for subsequent culturomics.

ABSTRACT: The discovery that the lung harbors a diverse microbiome, as revealed by next-generation sequencing, has significantly altered our understanding of respiratory health and disease. Despite the association between the lung microbiota and disease, the nature of their relationship remains poorly understood, and culture isolation of these microorganisms could help to determine their role in lung physiology. Current procedures for processing samples from the lower respiratory tract have been shown to affect the viability of microorganisms, so it is crucial to develop new methods to improve their survival. This study aimed to improve the isolation and characterization of lung microorganisms using a bead-beating homogenization method in a mouse model. Microsphere diameter and bead-beating time aff

SUBMITTER: Anduni L 

PROVIDER: S-EPMC11442450 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature