Exploring the anticancer mechanism of cardiac glycosides using proteome integral solubility alteration approach.

ABSTRACT:

Background and aims

Cardiac glycosides (CGs), traditionally used for heart failure, have shown potential as anti-cancer agents. This study aims to explore their multifaceted mechanisms in cancer cell biology using proteome integral solubility alteration (PISA), focusing on the interaction with key proteins implicated in cellular metabolism and mitochondrial function.

Methods

We conducted lysate-based and intact-cell PISA assays on cancer cells treated with CGs (Digoxin, Digitoxin, Ouabain) to analyze protein solubility changes. This was followed by mass spectrometric analysis and bioinformatics to identify differentially soluble proteins (DSPs). Molecular docking simulations were performed to predict protein-CG interactions. Public data including gene expression changes upon

SUBMITTER: Qin W 

PROVIDER: S-EPMC11442762 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature