BrightMice: a low-cost do-it-yourself instrument, designed for in vivo fluorescence mouse imaging.

ABSTRACT: In vivo fluorescent imaging represents a potent means for real-time probe quantification, facilitating insights into disease pathophysiology and therapeutic responses. Nonetheless, accurate signal quantification remains challenging due to inherent factors like light scattering and tissue absorption. Existing imaging systems, though sophisticated, often entail high costs and are typically restricted to well-funded laboratory settings. This study introduces BrightMice, an innovative in vivo fluorescent imaging system that harnesses 3D printing and consumer-grade digital cameras. Tailored for various fluorophores such as EYFP and E2-crimson, the system showcases both adaptability and effectiveness in detecting in vivo fluorescent signals in several reporter mouse strains. Comparative analyses

SUBMITTER: Boitet M 

PROVIDER: S-EPMC11442974 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature