Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines.

ABSTRACT: The International Mouse Phenotyping Consortium (IMPC) systematically produces and phenotypes mouse lines with presumptive null mutations to provide insight into gene function. The IMPC now uses the programmable RNA-guided nuclease Cas9 for its increased capacity and flexibility to efficiently generate null alleles in the C57BL/6N strain. In addition to being a valuable novel and accessible research resource, the production of 3313 knockout mouse lines using comparable protocols provides a rich dataset to analyze experimental and biological variables affecting in vivo gene engineering with Cas9. Mouse line production has two critical steps - generation of founders with the desired allele and germline transmission (GLT) of that allele from founders to offspring. A systematic evaluation of th

SUBMITTER: Elrick H 

PROVIDER: S-EPMC11443006 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature