The impact of extraction method and pollen concentration on community composition for pollen metabarcoding.

ABSTRACT:

Premise

Plants and pollinators closely interact with each other to form complex networks of species interactions. Metabarcoding of pollen collections has recently been proposed as an advantageous method for the construction of such networks, but the extent to which diversity and community analyses depend on the extraction method and pollen concentration used remains unclear.

Methods

In this study, we used a dilution series of two pollen mixtures (a mock community and pooled natural pollen loads from bumblebees) to assess the effect of mechanical homogenization and two DNA extraction kits (spin column DNA extraction kit and magnetic bead DNA extraction kit) on the detected pollen richness and community composition.

Results

All species were successfully detected using t

SUBMITTER: Devriese A 

PROVIDER: S-EPMC11443440 | biostudies-literature | 2024 Sep-Oct

REPOSITORIES: biostudies-literature