Identification of immunity-related genes distinctly regulated by Manduca sexta Spаtzle-1/2 and Escherichia coli peptidoglycan.

ABSTRACT: The immune system of Manduca sexta has been well studied to understand molecular mechanisms of insect antimicrobial responses. While evidence supports the existence of major immune signaling pathways in this species, it is unclear how induced production of defense proteins is specifically regulated by the Toll and Imd pathways. Our previous studies suggested that diaminopimelic acid-type peptidoglycans (DAP-PG) from Gram-negative and some Gram-positive bacteria, more than Lys-type peptidoglycans (Lys-PG) from other Gram-positive bacteria, triggers both pathways through membrane-bound receptors orthologous to Drosophila Toll and PGRP-LC. In this study, we produced M. sexta proSpätzle-1 and proSpätzle-2 in Sf9 cells, identified their processing enzymes, and used prophenoloxidase activating p

SUBMITTER: Miao Z 

PROVIDER: S-EPMC11443596 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature