Decellularized extracellular matrix derived from dental pulp stem cells promotes gingival fibroblast adhesion and migration.

ABSTRACT:

Background

Decellularized extracellular matrix (dECM) has been proposed as a useful source of biomimetic materials for regenerative medicine due to its biological properties that regulate cell behaviors. The present study aimed to investigate the influence of decellularized ECM derived from dental pulp stem cells (DPSCs) on gingival fibroblast (GF) cell behaviors. Cells were isolated from dental pulp and gingival tissues. ECM was derived from culturing dental pulp stem cells in growth medium supplemented with ascorbic acid. A bioinformatic database of the extracellular matrix was constructed using Metascape. GFs were reseeded onto dECM, and their adhesion, spreading, and organization were subsequently observed. The migration ability of the cells was determined using a scratch assay

SUBMITTER: Nowwarote N 

PROVIDER: S-EPMC11443845 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature