Probing the orthogonality and robustness of the mammalian RNA-binding protein Musashi-1 in Escherichia coli.

ABSTRACT: RNA recognition motifs (RRMs) are widespread RNA-binding protein domains in eukaryotes, which represent promising synthetic biology tools due to their compact structure and efficient activity. Yet, their use in prokaryotes is limited and their functionality poorly characterized. Recently, we repurposed a mammalian Musashi protein containing two RRMs as a translation regulator in Escherichia coli. Here, employing high-throughput RNA sequencing, we explored the impact of Musashi expression on the transcriptomic and translatomic profiles of E. coli, revealing certain metabolic interference, induction of post-transcriptional regulatory processes, and spurious protein-RNA interactions. Engineered Musashi protein mutants displayed compromised regulatory activity, emphasizing the importance of bo

SUBMITTER: Dolcemascolo R 

PROVIDER: S-EPMC11443895 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature