Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation.

ABSTRACT:

Background

Although several cell culture systems have been developed to investigate the function of the mammary gland in dairy livestock, they have potential limitations, such as the loss of alveolar structure or genetic and phenotypic differences from their native counterparts. Overcoming these challenges is crucial for lactation research. Development of protocols to establish lactating organoid of livestock represents a promising goal for the future. In this study, we developed a protocol to establish a culture system for mammary organoids in dairy goats to model the mammary gland development and lactation process.

Results

The organoids cultured within an extracellular matrix gel maintained a bilayer structure that closely resembled the native architecture of mammary tissu

SUBMITTER: Jia L 

PROVIDER: S-EPMC11443931 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature