New Bacillus paralicheniformis strain with high proteolytic and keratinolytic activity.

ABSTRACT: Bacillus paralicheniformis T7, which exhibits high proteolytic and keratinolytic activities, was isolated from soil in Kazakhstan. Its secreted proteases were thermostable and alkaline, demonstrating maximum activity at 70 °C and pH 9.0. The proteases and keratinases of this strain were sensitive to Ni²⁺, Co²⁺, Mn²⁺, and Cd²⁺, with Cu²⁺, Co²⁺ and Cd²⁺ negatively affecting keratinolytic activity, and Fe³⁺ ions have a strong inhibitory effect on proteolytic and keratinolytic activity. Seven proteases were identified in the enzymatic extract of B. paralicheniformis T7: four from the serine peptidase family and three from the metallopeptidase family. The proteases hydrolyzed 1 mg of casein, hemoglobin, gela

SUBMITTER: Aktayeva S 

PROVIDER: S-EPMC11444040 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature