A genetically encoded fluorescent heme sensor detects free heme in plants.

ABSTRACT: Heme is produced in plants via a plastid-localized metabolic pathway and is subsequently distributed to all cellular compartments. In addition to covalently and noncovalently bound heme, a comparatively small amount of free heme that is not associated with protein is available for incorporation into heme-dependent proteins in all subcellular compartments and for regulatory purposes. This "labile" fraction may also be toxic. To date, the distribution of the free heme pool in plant cells remains poorly understood. Several fluorescence-based methods for the quantification of intracellular free heme have been described. For this study, we used the previously described genetically encoded heme sensor 1 (HS1) to measure the relative amounts of heme in different plant subcellular compartments. In

SUBMITTER: Wen B 

PROVIDER: S-EPMC11444292 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature