Mechanism of enterovirus VP0 maturation cleavage based on the structure of a stabilised assembly intermediate.

ABSTRACT: Molecular details of genome packaging are little understood for the majority of viruses. In enteroviruses (EVs), cleavage of the structural protein VP0 into VP4 and VP2 is initiated by the incorporation of RNA into the assembling virion and is essential for infectivity. We have applied a combination of bioinformatic, molecular and structural approaches to generate the first high-resolution structure of an intermediate in the assembly pathway, termed a provirion, which contains RNA and intact VP0. We have demonstrated an essential role of VP0 E096 in VP0 cleavage independent of RNA encapsidation and generated a new model of capsid maturation, supported by bioinformatic analysis. This provides a molecular basis for RNA-dependence, where RNA induces conformational changes required for VP0 mat

SUBMITTER: Kingston NJ 

PROVIDER: S-EPMC11444389 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature