Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.

ABSTRACT: Engineered microorganisms have emerged as viable alternatives for limonene production. However, issues such as low enzyme abundance or activities, and regulatory feedback/forward inhibition may reduce yields. To understand the underlying metabolism, we adopted a systems biology approach for an engineered limonene-producing Escherichia coli strain K-12 MG1655. Firstly, we generated time-series metabolomics data and, secondly, developed a dynamic model based on enzyme dynamics to track the native metabolic networks and the engineered mevalonate pathway. After several iterations of model fitting with experimental profiles, which also included ¹³C-tracer studies, we performed in silico knockouts (KOs) of all enzymes to identify bottleneck(s) for optimal limonene yields. The simulati

SUBMITTER: Khanijou JK 

PROVIDER: S-EPMC11445242 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature