Lazy neutrophils - a lack of DGAT1 reduces the chemotactic activity of mouse neutrophils.

ABSTRACT:

Background

Neutrophils are key players in the innate immune system, actively migrating to sites of inflammation in the highly energetic process of chemotaxis. In this study, we focus on the role of acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1), an enzyme that catalyzes the synthesis of triglycerides, the major form of stored energy, in neutrophil chemotaxis.

Methods and results

Using a mouse model of psoriasis, we show that DGAT1-deficiency reduces energy-demanding neutrophil infiltration to the site of inflammation, but this inhibition is not caused by decreased glycolysis and reduced ATP production by neutrophils lacking DGAT1. Flow cytometry and immunohistochemistry analysis demonstrate that DGAT1 also does not influence lipid accumulation in lipid droplets during in

SUBMITTER: Uchanska A 

PROVIDER: S-EPMC11445369 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature