Inflammatory and genomic interactions within keratoconus susceptible patients: a nationwide registered case-control study.

ABSTRACT:

Purpose

This study aimed to investigate the association between variants in the interleukin (IL)-1 gene cluster and susceptibility to keratoconus (KC) in an Iranian population.

Methods

In the case group, there were 188 KC patients diagnosed by clinical findings and corneal tomography. The control group included all 205 healthy controls with no personal or family history of eye-related, metabolic, or immune system-related disease. Using the standard salting out extraction procedure, genomic DNA was isolated from peripheral blood leukocytes. The genotypes were determined by applying agarose gel electrophoresis for the IL-1RN 86 bp VNTR and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rs16944 and rs1143634.

Results

The results showed

SUBMITTER: Doroodgar F 

PROVIDER: S-EPMC11446043 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature