A single amplified genome catalog reveals the dynamics of mobilome and resistome in the human microbiome.

ABSTRACT:

Background

The increase in metagenome-assembled genomes (MAGs) has advanced our understanding of the functional characterization and taxonomic assignment within the human microbiome. However, MAGs, as population consensus genomes, often aggregate heterogeneity among species and strains, thereby obfuscating the precise relationships between microbial hosts and mobile genetic elements (MGEs). In contrast, single amplified genomes (SAGs) derived via single-cell genome sequencing can capture individual genomic content, including MGEs.

Results

We introduce the first substantial SAG dataset (bbsag20) from the human oral and gut microbiome, comprising 17,202 SAGs above medium-quality without co-assembly. This collection unveils a diversity of bacterial lineages across 312 oral and

SUBMITTER: Kawano-Sugaya T 

PROVIDER: S-EPMC11446047 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature