CyaY and TusA regulate ISC- and SUF-mediated l-cysteine desulfurase activity.

ABSTRACT: CyaY, the frataxin homolog of Escherichia coli, plays an important role in ISC iron-sulfur cluster assembly through interactions with the cysteine desulfurase IscS, which regulate the supply of sulfur. IscS is not exclusive for ISC Fe-S cluster assembly, as it functions as a hub for the supply of sulfur to a number of other sulfur-requiring pathways, such as for the biosynthesis of Moco and thiolated tRNAs. How the balance of sulfur supply to the various competing pathways is achieved is not fully understood, but a network of protein-protein interactions plays a key role. For example, IscU and TusA compete for binding to IscS and thus for sulfur supply to ISC and Moco/tRNA biosynthesis. Here, we show that TusA can displace CyaY from IscS and can form hetero-complexes involving IscS,

SUBMITTER: Olivieri P 

PROVIDER: S-EPMC11446229 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature