Construction of an ultra-strong PtacM promoter via engineering the core-element spacer and 5' untranslated region for versatile applications in <i>Corynebacterium glutamicum</i>.

ABSTRACT: As one of the most important synthetic biology elements in transcriptional regulation, promoters play irreplaceable roles in metabolic engineering. For the industrial microorganism Corynebacterium glutamicum, both the construction of a promoter library with gradient strength and the creation of ultra-strong promoters are essential for the production of target enzymes and compounds. In this work, the spacer sequence (both length and base) between the -35 and -10 regions, and the 5'-terminal untranslated region (5'UTR) were particularly highlighted to investigate their contributions to promoter strength. We constructed a series of artificially induced promoters based on the classical tac promoter using C. glutamicum ATCC13032 as the host. Here, we explored the effect of sequenc

SUBMITTER: Du Y 

PROVIDER: S-EPMC11446368 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature