4,5-Diamino-2-Thiouracil-Powered Dual-Mode Biosensor for Sensitive, Nonenzymatic Determination of Saliva Uric Acid Levels.

ABSTRACT: Nonenzymatic and rapid monitoring of uric acid levels is of great value for early diagnosis, prevention, and management of oxidative stress-associated diseases. However, fast, convenient, and low-cost uric acid detection remains challenging, especially in resource-limited settings. In this study, a novel and rapid biosensing approach was developed for the simultaneous visualization and quantification of uric acid levels by using the unique surface plasmon resonance and photothermal property of 4,5-diamino-2-thiouracil (DT)-capped gold nanoparticles (AuNPs). With the presence of uric acid, DT-capped AuNPs rapidly aggregated, and a visible color/photothermal change was used for uric acid quantification within 15 min. The limit of detection was determined to be 11.3 and 6.6 μM for the

SUBMITTER: Wu Z 

PROVIDER: S-EPMC11446619 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature