Cytosolic calcium regulates hepatic mitochondrial oxidation, intrahepatic lipolysis, and gluconeogenesis via CAMKII activation.

ABSTRACT: To examine the roles of mitochondrial calcium Ca²⁺ ([Ca²⁺]_mt) and cytosolic Ca²⁺ ([Ca²⁺]_cyt) in the regulation of hepatic mitochondrial fat oxidation, we studied a liver-specific mitochondrial calcium uniporter knockout (MCU KO) mouse model with reduced [Ca²⁺]_mt and increased [Ca²⁺]_cyt content. Despite decreased [Ca²⁺]_mt, deletion of hepatic MCU increased rates of isocitrate dehydrogenase flux, α-ketoglutarate dehydrogenase flux, and succinate dehydrogenase flux in vivo. Rates of [¹⁴C₁₆]palmitate oxidation and intrahepatic lipolysis were increased in MCU KO liver slices, which led to decreased hepatic triacylglycerol content. These effect

SUBMITTER: LaMoia TE 

PROVIDER: S-EPMC11446666 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature