Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating autophagy through promoting deubiquitination of adenosine 5'-monophosphate (AMP)-activated protein kinase α1 via ubiquitin specific peptidase 5.

ABSTRACT:

Background

Hydrogen sulfide (H₂S), the third gasotransmitter discovered, regulates a variety of physiological functions. Whether H₂S alleviates skeletal muscle ageing by regulating autophagy has not been reported.

Methods

Mice were administered 150 mg/kg/day of D-galactose (D-gal), and C2C12 myotubes were cultured in 20 g/L D-gal to induce ageing. Sodium hydrosulfide (NaHS) was employed as an exogenous donor in the treatment group. The intracellular concentration of H₂S was quantified by the 7-azido-4-methylcoumarin fluorescence probe. The proteins involved in the ubiquitin-mediated degradation of AMPKα1 were detected by liquid chromatography tandem mass spectrometry (LC-MS/MS) and co-immunoprecipitation (Co-IP). S-sulfhydration of USP5 was t

SUBMITTER: Yang JH 

PROVIDER: S-EPMC11446701 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature