A multi-omics approach to overeating and inactivity-induced muscle atrophy in db/db mice.

ABSTRACT:

Background

Overeating and inactivity are associated with type 2 diabetes. This study aimed to investigate its pathological basis using integrated omics and db/db/mice, a model representing this condition.

Methods

The study involved housing 8-week-old db/m and db/db mice for 8 weeks. Various analyses were conducted, including gene expression in skeletal muscle and small intestine using next-generation sequencing; cytokine arrays of serum; assessment of metabolites in skeletal muscle, stool, and serum; and analysis of the gut microbiota. Histone modifications in small intestinal epithelial cells were profiled using CUT&Tag.

Results

Compared with db/m mice, db/db mice had 22.4% lower grip strength and approximately five times the visceral fat weight (P < 0.0001). Serum c

SUBMITTER: Okamura T 

PROVIDER: S-EPMC11446703 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature