Lateral flow assay sensitivity and signal enhancement via laser µ-machined constrains in nitrocellulose membrane.

ABSTRACT: Lateral flow assay (LFA) is a handful diagnostic technology that can identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other common respiratory viruses in one strip, which can be tested at the point-of-care without the need for equipment or skilled personnel outside the laboratory. Although its simplicity and practicality make it an appealing solution, it remains a grand challenge to substantially enhance the colorimetric LFA sensitivity. In this work, we present a straightforward approach to enhance the sensitivity of LFA by imposing the flow constraints in nitrocellulose (NC) membranes via a number of vertical femtosecond laser micromachined microchannels which is important for prolonged specific binding interactions. Porous NC membrane surfaces were structured wi

SUBMITTER: Khatmi G 

PROVIDER: S-EPMC11446913 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature