Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation.

ABSTRACT:

Background

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract, but the molecular mechanisms underlying IBD are incompletely understood. In this study, we explored the role and regulating mechanism of otubain 2 (OTUB2), a deubiquitinating enzyme, in IBD.

Methods

To study the function of OTUB2 in IBD, we generated Otub2^-/- mice and treated them with dextran sulfate sodium (DSS) to induce experimental colitis. Bone marrow transplantation was performed to identify the cell populations that were affected by OTUB2 in colitis. The molecular mechanism of OTUB2 in signal transduction was studied by various biochemical methods.

Results

OTUB2 was highly expressed in colon-infiltrating macrophages in both humans with IBD a

SUBMITTER: Du X 

PROVIDER: S-EPMC11446981 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature