Development of novel canine phage display-derived neutralizing monoclonal antibody fragments against rabies virus from immunized dogs.

ABSTRACT: Animal rabies is a potentially fatal infectious disease in mammals, especially dogs. Currently, the number of rabies cases in pet dogs is increasing in several regions of Thailand. However, no passive postexposure prophylaxis (PEP) has been developed to combat rabies infection in animals. As monoclonal antibodies (MAbs) are promising biological therapies for postinfection, we developed a canine-neutralizing MAb against rabies virus (RABV) via the single-chain variable fragment (scFv) platform. Immunized phage-displaying scFv libraries were constructed from PBMCs via the pComb3XSS system. Diverse canine VHVLκ and VHVLλ libraries containing 2.4 × 10⁸ and 1.3 × 10⁶ clones, respectively, were constructed. Five unique clones that show binding affinity with the RABV glycopr

SUBMITTER: Chorpunkul A 

PROVIDER: S-EPMC11447112 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature