PD-L1⁺ neutrophils induced NETs in malignant ascites is a potential biomarker in HCC.

ABSTRACT:

Background

Since differentiating malignant ascites from benign ascites has always been a clinical difficult, recognition of novel biomarkers in malignant ascites of hepatocellular carcinoma (HCC) patients could be helpful for establishing a diagnosis for HCC patients with ascitic fluids.

Methods

Thirty-five HCC patients with malignant ascites and chronic liver diseases patients with benign ascites were enrolled. Serum and ascites specimens were collected to determine TAN subpopulations and NETs concentration. Then, the correlation between ascitic NETs levels and clinical features were analyzed, and ROC curves were generated to evaluate the diagnostic value of NETs. For in vitro study, fresh neutrophils were employed to explore the underlying mechanism of TAN polarization and

SUBMITTER: Sun X 

PROVIDER: S-EPMC11447185 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature