Quantitative proteomics combined independent PRM analysis reveals the mitochondrial and synaptic mechanism underlying norisoboldine's antidepressant effects.

ABSTRACT: Major depressive disorder (MDD) is a common disease affecting 300 million people worldwide. The existing drugs are ineffective for approximately 30% of patients, so it is urgent to develop new antidepressant drugs with novel mechanisms. Here, we found that norisoboldine (NOR) showed an antidepressant efficacy in the chronic social defeat stress (CSDS) depression model in the tail suspension, forced swimming, and sucrose consumption tests. We then utilized the drug-treated CSDS mice paradigm to segregate and gain differential protein groups of CSDS versus CON (CSDS_CON), imipramine (IMI)-treated versus CSDS (IMI_CSDS), and NOR-treated versus CSDS (NOR_CSDS) from the prefrontal cortex. These protein expression alterations were first analyzed by ANOVA with p < 0.

SUBMITTER: Li L 

PROVIDER: S-EPMC11447221 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature