Sustained alterations in proximal tubule gene expression in primary culture associate with HNF4A loss.

ABSTRACT: Primary cultures of proximal tubule cells are widely used to model the behavior of kidney epithelial cells in vitro. However, de-differentiation of primary cells upon culture has been observed and appreciated for decades, yet the mechanisms driving this phenomenon remain poorly understood. This confounds the interpretation of experiments using primary kidney epithelial cells and prevents their use to engineer functional kidney tissue ex vivo. In this report, we measure the dynamics of cell-state transformations in early primary culture of mouse proximal tubules to identify key pathways and processes that correlate with and may drive de-differentiation. Our data show that the loss of proximal-tubule-specific genes is rapid, uniform, and sustained even after confluent, polarized epithelial m

SUBMITTER: Telang AC 

PROVIDER: S-EPMC11447228 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature