Suspension culture on microcarriers and as aggregates enables expansion and differentiation of pluripotent stem cells (PSCs).

ABSTRACT:

Background aims

Human pluripotent stem cells (PSCs) hold a great promise for promoting regenerative medical therapies due to their ability to generate multiple mature cell types and for their high expansion potential. However, cell therapies require large numbers of cells to achieve desired therapeutic effects, and traditional two-dimensional static culture methods cannot meet the required production demand for cellular therapies. One solution to this problem is scaling up expansion of PSCs in bioreactors using culture strategies such as growing cells on microcarriers or as aggregates in suspension culture.

Methods

In this study, we directly compared PSC expansion and quality parameters in microcarrier- and aggregate-cultures grown in single-use vertical-wheel bioreactors.

SUBMITTER: Vallabhaneni H 

PROVIDER: S-EPMC11447601 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature