An Improved Method and Device for Nucleic Acid Isolation Using a High-Salt Gel Electroelution Trap.

ABSTRACT: The success of DNA analytical methods, including long-read sequencing, depends on the availability of high-quality, purified DNA. Previously, we developed a method and device for isolating high-molecular-weight (HMW) DNA for long-read sequencing using a high-salt gel electroelution trap. Here, we present an improved version of this method for purifying nucleic acids with high yield and purity from even the most challenging biological samples. The proposed method is a significant improvement over the previously published procedure, offering a simple, fast, and efficient solution for isolating HMW DNA and smaller DNA and RNA molecules. The method utilizes vertical gel electrophoresis in two nested, partially overlapping electrophoretic columns. The upper, smaller-diameter column has a thin l

SUBMITTER: Kalendar R 

PROVIDER: S-EPMC11447668 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature