Improving Reliability of Immunological Assays by Defining Minimal Criteria for Cell Fitness.

ABSTRACT: Human PBMC-based assays are often used as biomarkers for the diagnosis and prognosis of disease, as well as for the prediction and tracking of response to biological therapeutics. However, the development and use of PBMC-based biomarker assays is often limited by poor reproducibility. Complex immunological assays can be further complicated by variation in cell handling before analysis, especially when using cryopreserved cells. Variation in postthaw viability is further increased if PBMC isolation and cryopreservation are done more than a few hours after collection. There is currently a lack of evidence-based standards for the minimal PBMC viability or "fitness" required to ensure the integrity and reproducibility of immune cell-based assays. In this study, we use an "induced fail" approac

SUBMITTER: Ivison S 

PROVIDER: S-EPMC11447670 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature