Avoiding overflow metabolite formation in Komagataella phaffii fermentations to enhance recombinant protein production.

ABSTRACT:

Background

Komagataella phaffii (K. phaffii), formerly known as Pichia pastoris, is a widely utilized yeast for recombinant protein production. However, due to the formation of overflow metabolites, carbon yields may be reduced and product recovery becomes challenging. This study investigates the impact of oxygen availability, different glucose concentrations and feeding strategies on overflow metabolite formation and recombinant protein production in K. phaffii.

Results

High glucose concentrations in batch fermentation, as applied in literature, lead to substantial ethanol accumulation, adversely affecting biomass yield and product formation. Increasing dissolved oxygen setpoints does not significantly reduce ethanol formation, indicating that glucose surplus, rather than o

SUBMITTER: Steimann T 

PROVIDER: S-EPMC11448000 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature