Interplay between gut microbiota and tryptophan metabolism in type 2 diabetic mice treated with metformin.

ABSTRACT: Tryptophan (TRP) metabolites have been identified as potent biomarkers for complications of type 2 diabetes mellitus (T2DM). However, it remains unclear whether the therapeutic effect of metformin in T2DM is related to the modulation of TRP metabolic pathway. This study aims to investigate whether metformin affects TRP metabolism in T2DM mice through the gut microbiota. A liquid chromatography-tandem mass spectrometry method was established to determine 16 TRP metabolites in the serum, colon content, urine, and feces of T2DM mice, and the correlations between metabolites and the T2DM mice gut microbiota were performed. The method demonstrated acceptable linearity (R² > 0.996), with the limit of quantification ranging from 0.29 to 69.444 nmol/L for 16 analytes, and the lim

SUBMITTER: Xie Y 

PROVIDER: S-EPMC11448047 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature