Cultivation in long-term simulated microgravity is detrimental to pyocyanin production and subsequent biofilm formation ability of <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Pseudomonas aeruginosa forms aggregates known as biofilms. Previous studies have shown that when P. aeruginosa is cultivated in space, thicker and structurally different biofilms are formed than from those grown on Earth. We investigated how microgravity, simulated in a laboratory setting, influenced the growth, colonization, and virulence potentials of a P. aeruginosa PA14 wild-type strain, as well as two surface attachment-defective (sad) mutants altered at crucial biofilm-forming steps: flgK and pelA. Using high-aspect ratio rotating-wall vessel (HARV) bioreactors, P. aeruginosa bacteria were grown to stationary phase under prolonged (6 days) exposure to simulated microgravity or normal gravity conditions. After the exposure, the capacity

SUBMITTER: Chen KZM 

PROVIDER: S-EPMC11448113 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature