Analysis of the utility of a rapid vesicle isolation method for clinical strains of <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Pseudomonas aeruginosa, a pathogen capable of causing diseases ranging from mild to life-threatening, has a large arsenal of virulence factors. Notably, extracellular vesicles have emerged as significant players in the pathogenesis of this organism. However, the full range of their functions is still being studied, and difficulties related to vesicle purification (long protocols, low yields, and specialized instruments) have become a major obstacle for their characterization. In this context, the utility of rapid new methods of vesicle isolation from clinical strains is still unknown. Here, we analyze the utility of the ExoBacteria OMV isolation kit for a collection of clinical strains of P. aeruginosa. We first phenotypically characterized 15 P. aeruginosa stra

SUBMITTER: Henriquez T 

PROVIDER: S-EPMC11448148 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature