Enterohemorrhagic <i>Escherichia coli</i> (EHEC) disrupts intestinal barrier integrity in translational canine stem cell-derived monolayers.

ABSTRACT: This study addresses the gap in translatable in vitro models for investigating Enterohemorrhagic E. coli (EHEC) infections, particularly relevant to both canine and human health. EHEC is known to induce acute colitis in dogs, leading to symptoms like hemorrhagic diarrhea and hemolytic uremic syndrome, similar to those observed in humans. However, understanding the pathophysiology and developing treatment strategies have been challenging due to the lack of effective models that replicate the clinical disease caused by EHEC in both species. Our approach involved the development of colonoid-derived monolayers using intestinal tissues from healthy, client-owned dogs. These monolayers were exposed to EHEC, and the impact of EHEC was assessed through several techniques, including t

SUBMITTER: Nagao I 

PROVIDER: S-EPMC11448187 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature