Augmented pathogen detection in brain abscess using metagenomic next-generation sequencing: a retrospective cohort study.

ABSTRACT: Brain abscess is a severe infection characterized by the accumulation of pus within the brain parenchyma. Accurate identification of the causative pathogens is crucial for effective treatment and improved patient outcomes. This 10-year retrospective, single-center study aimed to compare the detection performance of conventional culture methods and metagenomic next-generation sequencing (mNGS) in brain abscess. We reviewed 612 patients diagnosed with brain abscess and identified 174 cases with confirmed etiology. The median age was 52 years, with 69.5% males. Culture tests predominately identified gram-positive bacteria, particularly Streptococcus spp. Gram-negative bacteria, including Klebsiella spp., were also detected. However, mNGS revealed a more diverse pathogen spectrum

SUBMITTER: Wang X 

PROVIDER: S-EPMC11448231 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature