Resolved genomes of wastewater ESBL-producing Escherichia coli and metagenomic analysis of source wastewater samples.

ABSTRACT: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli pose a serious threat to human health because of their resistance to the most commonly prescribed antibiotics: penicillins and cephalosporins. In this study, we provide a genomic and metagenomic context for the determinant beta-lactam resistance genes of ESBL-positive E. coli isolated from various wastewater treatment utilities in Oregon, USA. Class A beta-lactamase genes on chromosomes (blaCTX-M, blaTEM) were clustered with antibiotic resistance genes associated with other classes of antibiotics (sulfonamides and aminoglycosides) along with insertional elements. ESBL genes such as blaCTX-M, blaTEM, and blaSHV were also detected on conjugable plasmids of IncF and IncI incompa

SUBMITTER: Cheney C 

PROVIDER: S-EPMC11448238 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature