Tracking changes in functionality and morphology of repopulated microglia in young and old mice.

ABSTRACT:

Background

Microglia (MG) are myeloid cells of the central nervous system that support homeostasis and instigate neuroinflammation in pathologies. Single-cell RNA sequencing (scRNA-seq) revealed the functional heterogeneity of MG in mouse brains. Microglia are self-renewing cells and inhibition of colony-stimulating factor 1 receptor (CSF1R) signaling depletes microglia which rapidly repopulate. The functions of repopulated microglia are poorly known.

Methods

We combined scRNA-seq, bulk RNA-seq, immunofluorescence, and confocal imaging to study the functionalities and morphology of repopulated microglia.

Results

A CSRF1R inhibitor (BLZ-945) depleted microglia within 21 days and a number of microglia was fully restored within 7 days, as confirmed by TMEM119 staining an

SUBMITTER: Luczak-Sobotkowska ZM 

PROVIDER: S-EPMC11448401 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature