The environmental microbial retrieving assessment of cell-processing facilities for cell therapy in a hospital laboratory.

ABSTRACT: Cell therapy represents a promising treatment modality. A critical component in the production of cell therapy products is maintaining the sterility of cell therapy clean rooms (CTCRs). This study aimed to evaluate the environmental microbial load within CTCRs. We systematically monitored microbial load in CTCRs, following established guidelines. Cultured microbial samples underwent metagenomic sequencing, and alpha and beta diversity analyses, functional annotation, and resistance gene profiling were performed using various bioinformatics tools to assess microbial diversity and function. From November 2023 to January 2024, we collected 42 environmental microbial colony samples from various sources within the CTCR and performed metagenomic sequencing on 39 samples. Alpha diversity analysis

SUBMITTER: Lian J 

PROVIDER: S-EPMC11448444 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature