ASV vs OTUs clustering: Effects on alpha, beta, and gamma diversities in microbiome metabarcoding studies.

ABSTRACT: In microbial community sequencing, involving bacterial ribosomal 16S rDNA or fungal ITS, the targeted genes are the basis for taxonomical assignment. The traditional bioinformatical procedure has for decades made use of a clustering protocol by which sequences are pooled into packages of shared percent identity, typically at 97%, to yield Operational Technical Units (OTUs). Progress in the data processing methods has however led to the possibility of minimizing technical sequencers errors, which were the main reason for the OTU choice, and to analyze instead the exact Amplicon Sequence Variants (ASV) which is a choice yielding much less agglomerated reads. We have tested the two procedures on the same 16S metabarcoded bacterial amplicons dataset encompassing a series of samples from 17 adj

SUBMITTER: Fasolo A 

PROVIDER: S-EPMC11449282 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature