Cytoplasmic preassembly of the flagellar outer dynein arm complex in <i>Trypanosoma brucei</i>.

ABSTRACT: The outer dynein arm (ODA) is a large, multimeric protein complex essential for ciliary motility. The composition and assembly of ODA are best characterized in the green algae Chlamydomonas reinhardtii, where individual ODA subunits are synthesized and preassembled into a mature complex in the cytosol prior to ciliary import. The single-cellular parasite Trypanosoma brucei contains a motile flagellum essential for cell locomotion and pathogenesis. Similar to human motile cilia, T. brucei flagellum contains a two-headed ODA complex arranged at 24 nm intervals along the axonemal microtubule doublets. The subunit composition and the preassembly of the ODA complex in T. brucei, however, have not been investigated. In this study, we affinity-purified the ODA complex

SUBMITTER: Balasubramaniam K 

PROVIDER: S-EPMC11449384 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature