Clinical significance of acidic extracellular microenvironment modulated genes.

ABSTRACT:

Background

The extracellular pH (pH _e ) is known to be acidic. We investigated the effect of mild (pH _e 6.8) and severe (pH _e 5.9) acidosis on gene expression in mouse B16-BL6 melanoma cells using cDNA microarray analysis and compared them with the acidic pH _e dependence of human tumors.

Methods

B16-BL6 cells were treated with pH _e 7.4 (control), pH _e 6.8, and pH _e 5.9. The mRNA expression was analyzed by using the cDNA microarray. Heat map, volcano plot, and gene ontology enrichment analysis were performed. The data were compared with the gene signatures of published data GSE52031 and GSE8401 and compared with the pathological staging by GEPIA2, and

SUBMITTER: Kato Y 

PROVIDER: S-EPMC11449683 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature