Single-Cell Landscape and a Macrophage Subset Enhancing Brown Adipocyte Function in Diabetes.

ABSTRACT:

Backgruound

Metabolic dysregulation is a hallmark of type 2 diabetes mellitus (T2DM), in which the abnormalities in brown adipose tissue (BAT) play important roles. However, the cellular composition and function of BAT as well as its pathological significance in diabetes remain incompletely understood. Our objective is to delineate the single-cell landscape of BAT-derived stromal vascular fraction (SVF) and their characteristic alterations in T2DM rats.

Methods

T2DM was induced in rats by intraperitoneal injection of low-dose streptozotocin and high-fat diet feeding. Single-cell mRNA sequencing was then performed on BAT samples and compared to normal rats to characterize changes in T2DM rats. Subsequently, the importance of key cell subsets in T2DM was elucidated using vario

SUBMITTER: Gu J 

PROVIDER: S-EPMC11449828 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature