Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory.

ABSTRACT: CRISPR/Cas9 genome editing is a rapidly advancing technology that has the potential to accelerate research and development in a variety of fields. However, manual genome editing processes suffer from limitations in scalability, efficiency, and standardization. The implementation of automated systems for genome editing addresses these challenges, allowing researchers to cover the increasing need and perform large-scale studies for disease modeling, drug development, and personalized medicine. In this study, we developed an automated CRISPR/Cas9-based genome editing process on the StemCellFactory platform. We implemented a 4D-Nucleofector with a 96-well shuttle device into the StemCellFactory, optimized several parameters for single cell culturing and established an automated workflow for CR

SUBMITTER: Nießing B 

PROVIDER: S-EPMC11449837 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature