YTHDF3 modulates the progression of breast cancer cells by regulating FGF2 through m⁶A methylation.

ABSTRACT:

Introduction

Breast cancer (BC) is a prevailing malignancy among women, and its inconspicuous development contributes significantly to mortality. The RNA N6-methyladenosine (m⁶A) modification represents an emerging mechanism for gene expression regulation, with the active involvement of the YTH N6-methyladenosine RNA binding protein 3 (YTHDF3) in tumor progression across multiple cancer types. Nonetheless, its precise function in breast cancer necessitates further investigation.

Methods

The expression of YTHDF3 in both cell lines and patient tissues was examined using Western blotting, reverse transcription quantitative PCR (RT-qPCR), and immunohistochemistry (IHC) techniques. Bioinformatics analysis of methylated RNA immunoprecipitation sequencing (MeRIP-seq) and

SUBMITTER: Gong RF 

PROVIDER: S-EPMC11449838 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature