Decreased ATF5 level contributes to improved mitochondrial function in oocytes exposed to vitrification stress.

ABSTRACT:

Background

Mitochondrial unfolded protein response (mtUPR) plays an essential role in the response of mitochondria to stress-induced damage. Activating of transcription factor 5 (ATF5) can help to sustain mitochondrial function and regulate organelle recovery under mitochondrial stress. Vitrification is a stressor that disrupts mitochondrial activity and cell homeostasis. However, little is known about the function of ATF5 in response to the extreme biophysical and chemical stresses during oocyte vitrification.

Methods

The expression of ATF5 and mtUPR biomarkers were measured in fresh and vitrified oocytes. Subsequently, oocytes with ATF5 deficiency were constructed by siRNA microinjection, and the function of ATF5 in mitochondrial function and oocyte development were analyz

SUBMITTER: Zhou G 

PROVIDER: S-EPMC11449845 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature