SERS-based microdroplet platform for high-throughput screening of <i>Escherichia coli</i> strains for the efficient biosynthesis of D-phenyllactic acid.

ABSTRACT: D-Phenyllactic acid (D-PLA) is a potent antimicrobial typically synthesized through chemical methods. However, due to the complexity and large pollution of these reactions, a simpler and more eco-friendly approach was needed. In this study, a strain for D-PLA biosynthesis was constructed, but the efficiency was restricted by the activity of D-lactate dehydrogenase (DLDH). To address this issue, a DLDH mutant library was constructed and the Surface-Enhanced Raman Spectroscopy (SERS) was employed for the precise quantification of D-PLA at the single-cell level. The TB24 mutant exhibited a significant improvement in D-PLA productivity and a 23.03-fold increase in enzymatic activity, which was attributed to the enhanced hydrogen bonding and increased hydrophobicity within the substrate-binding

SUBMITTER: Hu L 

PROVIDER: S-EPMC11449890 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature