Dynamics of alternative polyadenylation in single root cells of Arabidopsis thaliana.

ABSTRACT:

Introduction

Single-cell RNA-seq (scRNA-seq) technologies have been widely used to reveal the diversity and complexity of cells, and pioneering studies on scRNA-seq in plants began to emerge since 2019. However, existing studies on plants utilized scRNA-seq focused only on the gene expression regulation. As an essential post-transcriptional mechanism for regulating gene expression, alternative polyadenylation (APA) generates diverse mRNA isoforms with distinct 3' ends through the selective use of different polyadenylation sites in a gene. APA plays important roles in regulating multiple developmental processes in plants, such as flowering time and stress response.

Methods

In this study, we developed a pipeline to identify and integrate APA sites from different scRNA-seq data

SUBMITTER: Bi X 

PROVIDER: S-EPMC11449893 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature