Cyclo(Pro-Tyr) elicits conserved cellular damage in fungi by targeting the [H⁺]ATPase Pma1 in plasma membrane domains.

ABSTRACT: Bioactive metabolites play a crucial role in shaping interactions among diverse organisms. In this study, we identified cyclo(Pro-Tyr), a metabolite produced by Bacillus velezensis, as a potent inhibitor of Botrytis cinerea and Caenorhabditis elegans, two potential cohabitant eukaryotic organisms. Based on our investigation, cyclo(Pro-Tyr) disrupts plasma membrane polarization, induces oxidative stress and increases membrane fluidity, which compromises fungal membrane integrity. These cytological and physiological changes induced by cyclo(Pro-Tyr) may be triggered by the destabilization of membrane microdomains containing the [H⁺]ATPase Pma1. In response to cyclo(Pro-Tyr) stress, fungal cells activate a transcriptomic and metabolomic response, which primarily involves lipid meta

SUBMITTER: Vela-Corcia D 

PROVIDER: S-EPMC11449911 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature